Diet induced obesity in zebra fish books

In four experiments, longevans rats ate ad libitum a synthetic semipurified diet containing 20 g hf or 4 g lowfat lf of fat100 g of diet or a nonpurified diet. Nutritional factors play important roles in the etiology of obesity, type 2 diabetes mellitus and their complications through genotype x environment interactions. These diets were based the ain93g diet and beef tallow was added to provide a highfat content. One group of zebrafish was overfed with artemia 60 mg dry weightday fish, a living prey consisting of a relatively high amount of fat. The relationship between highfat food consumption and obesity is wellestablished. Diet induced obese dio mouse models c57bl6ntac male. Gte decreased visceral adipose tissue vat volume induced by highfat diet in zebrafish larvae.

Different models of dietinduced obesity in zebrafish provided evidence that the pathophysiological pathways underlying dietinduced obesity in zebrafish are comparable to that of mammals, 14. Barry sears, to reduce the levels of dietinduced inflammation. An increase of visceral fat affects human bone health causing fragility, mechanical strength reduction, and increased propensity to fractures because of impaired bone matrix microstructure and aberrant bone cell function. Feeding hcd for 8 weeks resulted in exogenous obesity confirmed by higher body mass index bmi values of obese males 0. Future studies will enable us to understand the composite nature of mitochondrial flash activity and will encourage the generation of new indicators to better measure these phenomena. The burchells zebra prefer open woodland, scrub and grassland. Sucrose nonfermentingrelated kinase regulates both adipose. Effects of yuzu citrus junos siebold ex tanaka peel on the. Fatty diet is a significant factor causing obesity. Rats were fed a high fat diet and treated with a low dose of streptozotocin to create models of diet induced obesity or type 2 diabetes. Antiobesity mechanisms of pm in zebrafish and mice models of obesity. Highfat diet impairs cognitive function of zebrafish. Related publications genetic determinants of energy expenditure and insulin resistance in dietinduced obesity in mice.

Rat models of dietinduced obesity and high fatlow dose. The zone diet is a nutritional program developed by dr. The aim of this study was to examine the antiobesity effects of boiled tuna extract in c57bl6n mice with obesity induced by a highfat diet hfd. Body fat accumulation in zebrafish is induced by a diet rich. The effect of diet and exercise on intestinal integrity and microbial diversity in mice. We previously reported that snrk is ubiquitously and abundantly expressed in both white adipose tissue wat and brown adipose tissue bat, but snrk expression diminishes in adipose tissue in obesity. Compositions of real and mimetic cocoa are listed in table 2. International database resource for the laboratory mouse, providing integrated. Obesity and metabolic syndrome are associated with glomerulosclerosis and proteinuria, but the mechanisms are not known. Anti obesity mechanisms of pm in zebrafish and mice models of obesity.

Adult zebrafish were allocated to four diet groups and over 6 weeks. Although the zebrafish is a model that has become popular in recent decades for basic biomedical research due to the multiple advantages highlighted in several articles 40,41, it has not been used extensively for studies related to obesity and diet. Pdf dietinduced obesity in zebrafish shares common. Overfeedinginduced obesity in zebrafish shows similar symptoms to those in. The aim of this study is to examine the agerelated influence of hfdinduced obesity on mandibular bone architecture and. Dietinduced obesity in c57bl6j mice causes increased. Dietinduced obesity in male c57bl6 mice decreases fertility. Propensity to highfat dietinduced obesity in rats is. Zebrafish possess many structural and functional similarities with humans and have been used to model various human diseases, including a genetic model of.

Jun 24, 2012 backhed f, manchester jk, semenkovich cf, gordon ji 2007 mechanisms underlying the resistance to dietinduced obesity in germfree mice. Turnbaugh pj, ley re, mahowald ma, magrini v, mardis er, gordon ji. Dietinduced obesity in zebrafish shares common pathophysiological pathways with mammalian obesity article pdf available in bmc physiology 101. The zone diet has been consistently demonstrated in controlled clinical. Most of those studies compared lean and obese animals and were based on excessive overfeeding of adult zebrafish with their natural diet in. Highfat diet impairs cognitive function of zebrafish scientific reports. A novel wistar rat model of obesityrelated nonalcoholic. Short chain fatty acids prevent highfatdietinduced obesity. The dietinduced obesity model dio model is an animal model used to study obesity using animals that have obesity caused by being fed highfat or highdensity diets. The gi tract functions as a major immunological organ as it must maintain tolerance to commensal and dietary antigens while remaining responsive to pathogenic stimuli. The gut microbiota as an environmental factor that regulates fat storage. Hf and hfc diets induced hepatic steatosis in zebrafish larvae. To evaluate the capacity of gluten to retain chemicals, we prepared gluten granules containing eight types of chemicals with different log p ow values and immersed them in water. Consequently, zebrafish might serve as a good animal model for.

Metabolic and bone effects of highfat diet in adult zebrafish. Short chain fatty acids prevent highfat diet induced obesity in mice by regulating g proteincoupled receptors and gut microbiota. A novel protocol for the oral administration of test. We previously reported that snrk is ubiquitously and abundantly expressed in both white adipose tissue wat and brown adipose tissue bat, but snrk expression diminishes.

We determined the anti obesity effects of boiled tuna extract 100, 200, or 400 mgkg on the progression of hfd induced obesity for 10 weeks. Nov 29, 2012 carbohydratefree diet for 2 or 4 weeks following protocols. Highfat dietinduced obesity triggers alveolar bone loss and. Rats were fed a high fat diet and treated with a low dose of streptozotocin to create models of diet induced obesity or. Adult danio rerio zebrafish represents a powerful model to study both metabolic diseases and bone metabolism. In this study, we used a dietinduced obesity approach in zebrafish danio. Short chain fatty acids prevent highfatdietinduced obesity in mice by regulating g proteincoupled receptors and gut microbiota. International database resource for the laboratory mouse, providing integrated genetic, genomic, and biological data. Researchers now describe the first genetic model of obesity in a fish. Obesity is a multifactorial health problem resulting from genetic, environmental and behavioral factors. Effects of yuzu peel citrus junos siebold ex tanaka, yuzu pomace after hexane extraction, and auraptene on metabolic disorders in zebrafish with dietinduced obesity dio were evaluated.

Everyone knows that eating lean fish helps slim waistlines, but researchers have found a new way fish can help eliminate obesity. In this study, we examined the in vivo role of phf2 in mice. Zebrafish as an experimental model for the study of obesity. Dec 22, 2017 an increase of visceral fat affects human bone health causing fragility, mechanical strength reduction, and increased propensity to fractures because of impaired bone matrix microstructure and aberrant bone cell function. E2f8 promotes hepatic steatosis through fabp3 expression in. To determine whether this also occurs in larvae, zebrafish fed with hf diet and hfc diet were stained with oil red o. There were some differences between zebrafish and mice in how pm exerts antiobesity mechanisms. The aim of this study is to examine the agerelated influence of hfd induced obesity on mandibular bone architecture and the structure of the periodontium in. Dec 19, 2011 effects of yuzu citrus junos siebold ex tanaka peel on the diet induced obesity in a zebrafish model journal of functional foods, vol. Obesity is a multifactorial disorder influenced by genetic and environmental factors. Dietinduced obesity is linked to marked but reversible alterations in the mouse distal gut microbiome. Obesity is a multifactorial disorder influenced by genetic and. It is intended to mimic the most common cause of obesity in humans.

We developed a rat model of diet induced obesity that will be a powerful tool for assessment of this issue. Overfed diet induced obesity dio zebrafish exhibit increased body weight gain, hypertriglyceridemia, hepatic steatosis 23,24, and visceral adiposity 25,26. Fatrich diets not only induce obesity in humans but also make. Persistent impaired glucose metabolism in a zebrafish. Rnaseq based transcriptome analysis of the antiobesity effect of. Lipidomic profiling of liver tissue from obesityprone and. The jackson laboratory maintains an inventory of diet induced obesity dio models, and can place any strain on any fully customized diet so that your mice are ready when you need them. Zebrafish possess many structural and functional similarities with humans and have been used to model various human diseases, including a genetic model of obesity. Native to south asia, it is a popular aquarium fish, frequently sold under the trade name zebra danio and thus often called a tropical fish although both tropical and subtropical the zebrafish is an important and widely used vertebrate model organism in scientific. Hf or hfc diets exposure results in steatosis and hepatitis in adult fish and rodents models 25, 35.

Short chain fatty acids prevent highfatdietinduced. However, it is as yet unclear whether highfat diet hfd induced obesity in childhood and adolescence determines agerelated changes in jaw bone health. Native to south asia, it is a popular aquarium fish, frequently sold under the trade name zebra danio and thus often called a tropical fish although both tropical and subtropical. Hf and hfc dietsinduced hepatic steatosis in zebrafish larvae. The purpose of this study was to determine if there is altered renal lipid metabolism and increased expression of sterol regulatory elementbinding proteins srebps in a model of dietinduced obesity. Effects of glucose treatment up to 14 days on blood glucose levels. All materials tested exhibited anti obesity effects. They are very dependent on water and you will seldom see them more than 12 km from it.

Reactive oxygen species and oxidative stress in obesity. The diet contained 72% fat corn oil and lard, 28% protein, and 1% carbohydrate as energy content 17. Although adverse effects of obesity on female reproduction or oocyte development have been well recognized, its harmfulness to male fertility is still unclear because of reported. Research article a novel wistar rat model of obesity. Animal models of obesity are required to help us understand the signaling pathways underlying this condition. These cells were first recognized by elia metchnikoff in 1882 in the larvae of starfish upon insertion of thorns of tangerine tree and later in daphnia magna or common water flea infected with fungal spores as cells responsible for the process of phagocytosis of. E2f8 promotes hepatic steatosis through fabp3 expression. Obesity is a complex metabolic disease that is a serious detriment to both children and adult health, which induces a variety of diseases, such as cardiovascular disease, type ii diabetes, hypertension and cancer. We determined the antiobesity effects of boiled tuna extract 100, 200, or 400 mgkg on the progression of. We optimized protocols for generating dietinduced obese dio zebrafish model by feeding high cholesterol diet hcd.

Fatrich diets not only induce obesity in humans but also make animals obese. Yuzu peel significantly suppressed the rise in plasma triacylglycerol tg and liver lipid accumulation. Controlled highfat diet induces an obese syndrome in rats. High fat plus high cholesterol diet lead to hepatic steatosis. A novel protocol using gluten as a carrier material was developed to administer chemicals to adult zebrafish, per os p. The jackson laboratory maintains an inventory of dietinduced obesity dio models, and can place any strain on any fully customized diet so that your mice are ready when you need them. There were some differences between zebrafish and mice in how pm exerts anti obesity mechanisms. The purpose of this study was to establish a zebrafish model of dietinduced obesity dio. An obesityassociated gut microbiome with increased capacity for energy harvest. Hyperglycemia is the main feature for the diagnosis of this disease. Sucrose nonfermentingrelated kinase regulates both. Typically mice, rats, dogs, or nonhuman primates are used in these models. The gastrointestinal gi microbiota is the collection of microbes which reside in the gi tract and represents the largest source of nonself antigens in the human body. This was an extremely high fat diet for mice, enough to render them obese and or diabetic in 4 weeks.

In this study, it was purposed to examine histological effects of fatty diet induced obesity on liver from female obese rats. Our results suggest that a diet rich in fat, instead of protein or carbohydrate, induced body fat accumulation in zebrafish with mechanisms that might be similar to those in mammals. In this study, we found that pacific dulse, palmaria mollis pm, showed anti obesity effects in diet induced obesity models. Dietinduced adipocyte number increase in adult rats.

Research article open access dietinduced obesity in. The pathogenesis of nonalcoholic fatty liver disease nafld is not fully understood, and experimental models are an alternative to study this issue. Diet induced obese dio mouse models c57bl6ntac male mice. Effects of yuzu peel citrus junos siebold ex tanaka, yuzu pomace after hexane extraction, and auraptene on metabolic disorders in zebrafish with diet induced obesity dio were evaluated. We investigated the effects of a simple carbohydraterich diet on the development of obesity related nafld and the impact of physical training on the metabolic abnormalities associated with this disorder.

Larvae fed with control diet rarely developed steatosis after 7 and 10 days feeding 0 % and 11 %, respectively. All materials tested exhibited antiobesity effects. We generated phf2 floxed mice, systemic phf2 null mice by crossing phf2 floxed mice with cmvcre transgenic mice, and tamoxifen. This model has lead to many discoveries of the important signalings in obesity, such as akt and mtor. Backhed f, ding h, wang t, hooper lv, koh gy, nagy a, et al.

Highfat diet induced obesity in animal models niloofar hariri and louise thibault school of dietetics and human nutrition, mcgill university, quebec, canada epidemiological studies have shown a positive relationship between dietary fat intake and obesity. The first diet induced obese dio zebrafish model was reported in 2010 oka et al. Basic nutritional investigation ingested cocoa can prevent. We carried out physiological tests, plasma lipid assays, obesity measures, liver.

Body fat accumulation in zebrafish is induced by a diet. Backhed f, manchester jk, semenkovich cf, gordon ji 2007 mechanisms underlying the resistance to dietinduced obesity in germfree mice. Jun 19, 2007 everyone knows that eating lean fish helps slim waistlines, but researchers have found a new way fish can help eliminate obesity. In this study, we found that pacific dulse, palmaria mollis pm, showed antiobesity effects in dietinduced obesity models. Dietinduced dysbiosis of the intestinal microbiota and. High fat plus high cholesterol diet lead to hepatic. The first dietinduced obese zebrafish model dio was reported in 2010 through. In this paper, hyperglycemic zebrafish, when immersed in a 111 mm glucose solution for 14 days, developed increased glycation of proteins. The cape mountain specie prefers the mountainous areas of the eastern and western cape in southern africa. Effects of yuzu citrus junos siebold ex tanaka peel on.

Epigenetic regulation of adipogenesis by phf2 histone. Stern, and jules hirsch rockefeller university, new york, new york 10021 faust, irving m. Mar 21, 2017 different models of dietinduced obesity in zebrafish provided evidence that the pathophysiological pathways underlying dietinduced obesity in zebrafish are comparable to that of mammals, 14. These data show that hyperglycemia can be induced and maintained in zebrafish. Can a high fat paleo diet cause obesity and diabetes. Novel antiobesity properties of palmaria mollis in. Less than 5% of chemicals were eluted from gluten granules.

Zeigler zebrafish diet, 1lb bag during this covid19 pandemic, pentair global operations and supply teams are working diligently to help ensure our valued customers are getting the best possible service and delivery during this time. Diabetes mellitus dm affects over 10% of the worlds population. These cells were first recognized by elia metchnikoff in 1882 in the larvae of starfish upon insertion of thorns of tangerine tree and later in daphnia magna or common water flea infected with fungal spores as cells responsible for the process of. A particularly interesting aspect of obesity is the differences observed in response to the. May 20, 2015 in light of these similarities to mammals and the advantages of the system, the zebrafish model has been used in the field of lipid metabolism research for studies of lipidrelated diseases, including dyslipidaemia with hepatic steatosis induced by diet induced obesity dio 15, 16, atherosclerosis induced by highcholesterol diet and in a. The diet induced obesity model dio model is an animal model used to study obesity using animals that have obesity caused by being fed highfat or highdensity diets. Although zebrafish larvae have been used in genetic and chemical screening experiments to identify novel genes involved in the regulation of energy homeostasis and potential therapeutic targets to treat obesity,23, it remains unclear whether zebrafish can be used as a model for diet induced obesity dio, similar to that observed in mammals. The organs that show signs of adiposity, such as fat accumulation in the liver and visceral adipose tissues, are similar to those found in humans.

Dietinduced obesity models diets high in fat have been attributed to a host of diseases, including obesity, metabolic and inflammatory diseases. Childhood obesity and the zebrafish as a model for the. Sucrose nonfermentingrelated kinase snrk is a member of the ampkrelated kinase family, and its physiological role in adipose energy homeostasis and inflammation remains unknown. The present study was performed to investigate the molecular mechanism of 6gingerol on adipocytemediated systemic inflammation in vitro and in highfat dietinduced obese zebra fish. Nov 23, 2015 obesity is a multifactorial health problem resulting from genetic, environmental and behavioral factors. Since rats and mice show a similar relationship, they are considered an appropriate model. Dietinduced obesity in c57bl6j mice causes increased renal. We have characterised molecular adaptation to high fat diet hfd feeding in inbred mouse strains widely used in genetic and physiological studies. Highfat dietinduced obesity in animal models niloofar hariri and louise thibault school of dietetics and human nutrition, mcgill university, quebec, canada epidemiological studies have shown a positive relationship between dietary fat intake and obesity. Nutrigenomics of high fat diet induced obesity in mice.

Oct 21, 2010 obesity is a multifactorial disorder influenced by genetic and environmental factors. Zebrafish is emerging as an important model for studying obesity and related. Thus, zebrafish are a useful model of human dietinduced obesity, which. It is known that obesity is associated with many chronic disorders such as nonalcoholic fatty liver disease nafld. Macrophages are ubiquitously present innate immune cells in humans and animals belonging to both invertebrates and vertebrates. We examined whether reversal of high fat diet, stimulating weight loss, compared to two treatments previously shown to have beneficial effects, could improve glucose utilization and peripheral neuropathy in animal models of obesity and type 2 diabetes. Rats were examined for effects of cocoa ingestion with highfat diets. We carried out physiological tests, plasma lipid assays, obesity. Adult zebrafish have also been used as an obesity model.

We investigated the effects of a simple carbohydraterich diet on the development of obesityrelated nafld and the impact of physical training on the metabolic abnormalities associated with this disorder. The zebrafish danio rerio is a freshwater fish belonging to the minnow family of the order cypriniformes. Larvae fed with control diet rarely developed steatosis after 7 and 10 days feeding 0 %. The purpose of this study was to determine if there is altered renal lipid metabolism and increased expression of sterol regulatory elementbinding proteins srebps in a model of diet induced obesity.

Diet induced obesity models diets high in fat have been attributed to a host of diseases, including obesity, metabolic and inflammatory diseases. An eaters guide to a healthy microbiome human food project. Proofofconcept using pharmacokinetics and pharmacological evaluation of carbamazepine. The aim of this study was to examine the anti obesity effects of boiled tuna extract in c57bl6n mice with obesity induced by a highfat diet hfd. In light of these similarities to mammals and the advantages of the system, the zebrafish model has been used in the field of lipid metabolism research for studies of lipidrelated diseases, including dyslipidaemia with hepatic steatosis induced by dietinduced obesity dio 15, 16, atherosclerosisinduced by highcholesterol diet and in a. The purpose of this study was to establish a zebrafish model of diet induced obesity dio. Dietinduced obesity in zebrafish shares common pathophysiological pathways. Antiobesity effects of boiled tuna extract in mice with. Although adverse effects of obesity on female reproduction or oocyte development have been well recognized, its harmfulness to male fertility is still unclear. However, it is as yet unclear whether highfat diet hfdinduced obesity in childhood and adolescence determines agerelated changes in jaw bone health.